The use of safe and efficacious insecticides against the adult and larval populations of mosquito vectors is one of the most effective ways to rapidly interrupt transmission of Zika virus, as well as other viruses transmitted by Aedes mosquitoes such as chikungunya and dengue.

Insecticide resistance monitoring in field populations of Aedes is required to determine the levels, mechanisms and geographical distribution of resistance in order to select appropriate insecticides for vector control. Evidence-based decisions will ensure that effective insecticides are selected and used. Changes in insecticide susceptibility status should also direct policy and operational decisions.

This document summarizes WHO test procedures for the detection of insecticide resistance in Aedes larvae and adults including insect growth regulators (IGRs) and Bti products. It also outlines strategies to manageinsecticide resistance in countries facing Zika virus and other viruses transmitted by this species of mosquito.

This document is for qualified entomologists at national and sub-national level who are responsible for evaluating the susceptibility status of local Aedes populations.

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